5LXS
Tubulin-KS-1-199-32 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.580, 157.800, 179.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.226 - 2.200 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.22690 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2875: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.226 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.229 | 4.116 |
| Number of reflections | 150705 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 12.9 |
| CC(1/2) | 0.998 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0-3% PEG, 1-6% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |
