5LXO
Coiled-coil protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9681 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.250, 76.460, 86.891 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 28.964 - 2.179 |
| R-factor | 0.2352 |
| Rwork | 0.233 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lxn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS (Nov 3, 2014) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.964 | 2.240 |
| High resolution limit [Å] | 2.179 | 2.180 |
| Rmerge | 0.094 | 0.667 |
| Number of reflections | 37534 | |
| <I/σ(I)> | 21 | 2.3 |
| Completeness [%] | 95.5 | 67.8 |
| Redundancy | 13.1 | 7.3 |
| CC(1/2) | 0.999 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 35 % PEG 400, 0.05 M Sodium sulphate, 0.05 M Lithium sulphate, 0.05 M Tris pH 8.5 |
