5LXN
Coiled-coil protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97957, 0.97972, 0.96810, 0.99132 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.389, 76.780, 86.832 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 28.759 - 2.080 |
| R-factor | 0.2291 |
| Rwork | 0.227 |
| R-free | 0.28540 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.795 |
| Data reduction software | XDS (November 4, 2014) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | SHELXCD (0.3.i-beta) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.760 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.080 | 0.715 |
| Number of reflections | 37552 | |
| <I/σ(I)> | 22.4 | 2.7 |
| Completeness [%] | 82.5 | 33.8 |
| Redundancy | 13.8 | 9.2 |
| CC(1/2) | 1.000 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 35 % PEG 400, 0.05 M Sodium sulphate, 0.05 M Lithium sulphate, 0.05 M Tris pH 8.5 |
