5LX1
Cys-Gly dipeptidase GliJ mutant D304A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.330, 99.330, 106.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.18099 |
| Rwork | 0.179 |
| R-free | 0.22007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lx0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.453 |
| Number of reflections | 17088 | |
| <I/σ(I)> | 16.6 | 3.4 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 10% PEG6000 |
