5LWZ
Cys-Gly dipeptidase GliJ (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.800, 100.250, 106.640 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.1686 |
| Rwork | 0.167 |
| R-free | 0.19721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.044 | 0.481 |
| Number of reflections | 103656 | |
| <I/σ(I)> | 13.8 | 2.1 |
| Completeness [%] | 97.7 | 97.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0, 5% isopropanol |
