5LWY

Revised crystal structure of the human adiponectin receptor 2 in complex with a C18 free fatty acid

Replaces:  3WXW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-22
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	74.580, 101.030, 108.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.520 - 2.400
R-factor	0.199
Rwork	0.198
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3WXW
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	101.030
High resolution limit [Å]	2.400
Number of reflections	32174
<I/σ(I)>	8.55
Completeness [%]	98.1
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	100mM Na-Citrate, 400mM K-Citrate, 30% PEG400, pH 6.0, Lipidic mesophase method, temperature 293K