5LWW
Crystal structure of a laccase-like multicopper oxidase McoG from Aspergillus niger bound to zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.505 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.446, 128.158, 134.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gw0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP (10.2.23) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.398 | 2.810 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.128 | 0.452 |
| Number of reflections | 42878 | |
| <I/σ(I)> | 10.79 | 2.92 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 296 | 20% PEG3000, 0.2 M zinc acetate, 0.1 M imidazole pH=8.0 |
