5LWA

TURNIP YELLOW MOSAIC VIRUS PROTEASE/DEUBIQUITINASE DOMAIN, I847A MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	132.722, 39.677, 72.699
Unit cell angles	90.00, 121.97, 90.00

Refinement procedure

Resolution	37.421 - 1.653
R-factor	0.1716
Rwork	0.169
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lw5
RMSD bond length	0.006
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.712
High resolution limit [Å]	1.653	1.653
Rmerge	0.063	0.933
Number of reflections	38202
<I/σ(I)>	11.8
Completeness [%]	98.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M trisodium citrate pH 5.6, 0.2 M ammonium acetate, 18%(w/v) PEG 4000, 5%(v/v) MPD