5LW5

TURNIP YELLOW MOSAIC VIRUS PROTEASE/DEUBIQUITINASE DOMAIN, DELTAC5 MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	133.460, 39.695, 72.864
Unit cell angles	90.00, 122.13, 90.00

Refinement procedure

Resolution	37.452 - 1.649
R-factor	0.2029
Rwork	0.200
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a5u
RMSD bond length	0.005
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.708
High resolution limit [Å]	1.649	1.649
Rmerge	0.062	0.624
Number of reflections	39006
<I/σ(I)>	11.2
Completeness [%]	99.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M trisodium citrate pH 5.6, 0.2 M ammonium acetate, 15%(w/v) PEG 4000, 5%(v/v) MPD