5LW3
Azotobacter vinelandii Mannuronan C-5 epimerase AlgE6 A-module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-01 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.884, 63.672, 73.075 |
Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
Resolution | 44.617 - 1.190 |
R-factor | 0.1446 |
Rwork | 0.144 |
R-free | 0.16030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pyg |
RMSD bond length | 0.017 |
RMSD bond angle | 1.620 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.617 | 1.230 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.685 | |
Rmeas | 0.063 | 0.780 |
Number of reflections | 146496 | |
<I/σ(I)> | 12.31 | 1.8 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 100 mM BisTrisPropane pH 9.5, 15-30% PEG 8000 |