5LW2
Crystal structure of DARPin 5m3_D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000040 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.130, 28.000, 52.730 |
| Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
| Resolution | 46.527 - 1.750 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SVX chain A |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.452 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.527 | 1.813 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.105 | 1.654 |
| Number of reflections | 14009 | |
| <I/σ(I)> | 8.85 | 0.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.997 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 1000 10% PEG 8000 10% Ca-acetate 0.2 M Tris(HOAc) 0.1 M pH 7.5 |
