5LVV
Human OGT in complex with UDP and fused substrate peptide (Tab1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.533, 98.533, 367.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.700 - 2.540 |
| R-factor | 0.19599 |
| Rwork | 0.193 |
| R-free | 0.24466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pe4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.783 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.130 | |
| Number of reflections | 36043 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 3.5M sodium formate, 0.1M Tris pH 8.5 |
