5LVO
Human PDK1 Kinase Domain in Complex with Allosteric Compound PSE10 Bound to the PIF-Pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.220, 44.420, 47.870 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 72.541 - 1.090 |
| R-factor | 0.1363 |
| Rwork | 0.135 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hrc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.702 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 73.000 |
| High resolution limit [Å] | 1.090 |
| Number of reflections | 126722 |
| <I/σ(I)> | 15 |
| Completeness [%] | 99.7 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.2 M NA CITRATE, 0.1 M HEPES PH 7.5, 0.01 M DTT |
