5LVE

STRUCTURE OF THE VARIABLE DOMAIN OF HUMAN IMMUNOGLOBULIN K-4 LIGHT CHAIN LEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06
Detector	RIGAKU
Spacegroup name	P 62 2 2
Unit cell lengths	106.300, 106.300, 45.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
Rwork	0.186
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LVE WITH GLN89 CHANGED TO ALA
RMSD bond length	0.005
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.074	0.242
Number of reflections	7471
<I/σ(I)>	25	6.8
Completeness [%]	97.0	88.3
Redundancy	10	15.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		2.0 M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 1MM ZINC ACETATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8 (M)
3	1	reservoir	sodium HEPES	0.1 (M)
4	1	reservoir	zinc acetate	0.001 (M)