5LUT
Structures of DHBN domain of Gallus gallus BLM helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9754 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.686, 230.804, 50.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.774 - 2.720 |
| R-factor | 0.2387 |
| Rwork | 0.236 |
| R-free | 0.28290 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((dev_2650: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.302 | |
| High resolution limit [Å] | 2.718 | 2.815 |
| Rmerge | 0.114 | 1.855 |
| Number of reflections | 25224 | |
| <I/σ(I)> | 18.48 | 2.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 12.5 |
| CC(1/2) | 0.999 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1M MgCl2 0.1M Na-Citrate 12% PEG 1500 15% Glycerol |
