5LUT

Structures of DHBN domain of Gallus gallus BLM helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9754
Spacegroup name	P 21 21 2
Unit cell lengths	76.686, 230.804, 50.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.774 - 2.720
R-factor	0.2387
Rwork	0.236
R-free	0.28290
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX ((dev_2650: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.302
High resolution limit [Å]	2.718	2.815
Rmerge	0.114	1.855
Number of reflections	25224
<I/σ(I)>	18.48	2.23
Completeness [%]	100.0	100
Redundancy	11.6	12.5
CC(1/2)	0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	0.1M MgCl2 0.1M Na-Citrate 12% PEG 1500 15% Glycerol