5LUS
Structures of DHBN domain of Pelecanus crispus BLM helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9754 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.188, 72.408, 79.234 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 36.200 - 1.433 |
| R-factor | 0.1578 |
| Rwork | 0.157 |
| R-free | 0.17170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.239 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | |
| High resolution limit [Å] | 1.433 | 1.484 |
| Rmerge | 0.034 | 0.347 |
| Number of reflections | 105436 | |
| <I/σ(I)> | 18.56 | 2.26 |
| Completeness [%] | 83.0 | 54 |
| Redundancy | 3.2 | 2.4 |
| CC(1/2) | 0.998 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 298 | 0.2M NH4 Acetate 0.1M Na Citrate 16% PEG 3350 |
