5LUN
Ethylene Forming Enzyme from Pseudomonas syringae pv. phaseolicola - P1 ultra-high resolution crystal form in complex with iron, N-oxalylglycine and arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.839, 79.056, 97.859 |
| Unit cell angles | 91.56, 93.42, 100.80 |
Refinement procedure
| Resolution | 77.597 - 1.080 |
| R-factor | 0.1631 |
| Rwork | 0.162 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lsq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.600 | 1.120 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.053 | 0.822 |
| Number of reflections | 584165 | |
| <I/σ(I)> | 7.7 | 1.4 |
| Completeness [%] | 93.0 | 89.3 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.994 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% polyethylene glycol, 0.1 M Tris, 0.2 M sodium chloride, 0.01 M N-oxalylglycine, 0.02 M L-Arginine, protein concentration 15mg/mL |
