5LTR

Structure of the Yellow-Green Fluorescent Protein mNeonGreen from Branchiostoma lanceolatum at the near physiological pH 8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.953724
Spacegroup name	P 65 2 2
Unit cell lengths	72.270, 72.270, 153.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.560 - 1.210
R-factor	0.15931
Rwork	0.158
R-free	0.18415
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltp
RMSD bond length	0.009
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.560		1.240
High resolution limit [Å]	1.210	5.410	1.210
Rmerge	0.086	0.036	1.739
Number of reflections	72944
<I/σ(I)>	18.84	66.09	1.98
Completeness [%]	99.9	99.7	99.9
Redundancy	18.2
CC(1/2)	1.000	0.999	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG 8,000, 100mM HEPES