5LT4
nucleotide-free kinesin-1 motor domain T92V mutant, P1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.145, 102.108, 102.137 |
| Unit cell angles | 119.49, 91.95, 91.85 |
Refinement procedure
| Resolution | 49.277 - 2.881 |
| R-factor | 0.2085 |
| Rwork | 0.205 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lnu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | |
| High resolution limit [Å] | 2.870 | |
| Rmeas | 0.320 | 1.680 |
| Number of reflections | 43981 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 96.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM Mes pH 6.5 0.16 M ammonium sulfate 30% mPEG 5000 |
