5LSL

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	44.888, 47.776, 88.019
Unit cell angles	89.89, 83.61, 85.57

Refinement procedure

Resolution	44.470 - 1.650
R-factor	0.1963
Rwork	0.195
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mdf
RMSD bond length	0.014
RMSD bond angle	1.628
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	BALBES
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.470	44.470	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.077	0.039	0.338
Number of reflections	84322
<I/σ(I)>	13.3
Completeness [%]	96.7	98.5	95
Redundancy	2.3	3.9	1.9
CC(1/2)	0.988	0.998	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4