5LSB
Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | P 32 |
Unit cell lengths | 142.083, 142.083, 40.355 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.520 - 2.700 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lsl 1cvj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.406 |
Data reduction software | XDS (2012-09-26) |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.520 | 35.520 | 2.830 |
High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
Rmerge | 0.149 | 0.047 | 0.625 |
Rmeas | 0.171 | ||
Rpim | 0.083 | ||
Total number of observations | 104708 | ||
Number of reflections | 25001 | ||
<I/σ(I)> | 13 | ||
Completeness [%] | 99.9 | 98.5 | 100 |
Redundancy | 4.2 | 3.9 | 4.2 |
CC(1/2) | 0.984 | 0.996 | 0.677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9 |