5LSB

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.931
Spacegroup name	P 32
Unit cell lengths	142.083, 142.083, 40.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.520 - 2.700
R-factor	0.1862
Rwork	0.184
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lsl 1cvj
RMSD bond length	0.009
RMSD bond angle	1.406
Data reduction software	XDS (2012-09-26)
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.520	35.520	2.830
High resolution limit [Å]	2.700	8.960	2.700
Rmerge	0.149	0.047	0.625
Rmeas	0.171
Rpim	0.083
Total number of observations	104708
Number of reflections	25001
<I/σ(I)>	13
Completeness [%]	99.9	98.5	100
Redundancy	4.2	3.9	4.2
CC(1/2)	0.984	0.996	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9