5LS2

Receptor mediated chitin perception in legumes is functionally seperable from Nod factor perception

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	45.290, 130.690, 53.630
Unit cell angles	90.00, 107.70, 90.00

Refinement procedure

Resolution	29.218 - 2.300
R-factor	0.1862
Rwork	0.183
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eby
RMSD bond length	0.002
RMSD bond angle	0.451
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.218	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.069	0.860
Number of reflections	26292
<I/σ(I)>	15.07	2.2
Completeness [%]	100.0	100
Redundancy	4.2	4.3
CC(1/2)	0.998	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	0.2M ammonium sulfate, 28% peg 2000 MME, 0.1 M sodium acetate