5LRP

Mopeia Virus Exonuclease domain complexed with Magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	173
Detector technology	PIXEL
Collection date	2015-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9677
Spacegroup name	P 1 2 1
Unit cell lengths	45.439, 38.182, 137.093
Unit cell angles	90.00, 91.99, 90.00

Refinement procedure

Resolution	45.410 - 1.941
R-factor	0.1973
Rwork	0.195
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q7b
RMSD bond length	0.009
RMSD bond angle	1.223
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XDS
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.410	2.010
High resolution limit [Å]	1.941	1.941
Number of reflections	35271
<I/σ(I)>	14.88
Completeness [%]	99.7	98.28
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.1 M MES pH 6.5, 25 % (M/W) PEG 8000