5LR8
Structure of plastidial phosphorylase Pho1 from Barley
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.210, 63.480, 148.820 |
| Unit cell angles | 90.00, 115.21, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.21824 |
| Rwork | 0.217 |
| R-free | 0.25732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zb2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.143 | 1.114 |
| Number of reflections | 51731 | |
| <I/σ(I)> | 8.8 | 1.3 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.996 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 0.1M Na Citrate 30% PEG 4000 |
