5LR5
N6-methyladenine is accommodated in a conventional A-U basepair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9193 |
| Spacegroup name | P 65 |
| Unit cell lengths | 50.470, 50.470, 108.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.708 - 2.400 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.28730 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 4.691 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.710 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.143 | 2.700 |
| Number of reflections | 5911 | |
| <I/σ(I)> | 13.3 | 1.4 |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 41.7 | 38.7 |
| CC(1/2) | 1.000 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Sulfate 0.1 M Sodium Acetate trihydrate pH 4.6 25% w/v Polyethylene Glycol 4000 |
