5LQT
RNA duplex has central consecutive GA pairs flanked by G-C basepairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9193 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 34.340, 34.340, 103.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.583 - 1.500 |
| R-factor | 0.2205 |
| Rwork | 0.220 |
| R-free | 0.22730 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 4.259 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.350 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 2.080 |
| Number of reflections | 10522 | |
| <I/σ(I)> | 21.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 12.4 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.02 M Calcium Chloride dihydrate, 0.1 M Sodium Acetate trihydrate pH 4.6, 30% v/v 2-Methyl-2,4-pentanediol |
