5LQI
W288A mutant of GlxA from Streptomyces lividans: apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.543, 126.294, 106.939 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.920 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4unm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.063 | 0.640 |
| Number of reflections | 99551 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 99.7 | 94.9 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.997 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | Equal volumes of protein (15 mg ml-1) and reservoir solution (20 % w/v 20 K polyethylene glycol, 0.1 M sodium acetate, pH 4) |
