5LQ7
Salmonella effector SpvD - G161 variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.000, 51.970, 47.220 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 44.980 - 1.600 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lq6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.410 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.970 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.104 | |
| Number of reflections | 26737 | |
| <I/σ(I)> | 10.4 | 1.9 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 5.9 | 6 |
| CC(1/2) | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Tris, 200 mM NaCl, 22 % PEG3350 |
