5LQ2

Crystal structure of Tyr24 phosphorylated Annexin A2 at 3.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	61.370, 99.200, 173.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.510 - 3.400
R-factor	0.19871
Rwork	0.197
R-free	0.23403
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hyw
RMSD bond length	0.011
RMSD bond angle	1.402
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.510	3.520
High resolution limit [Å]	3.400	3.400
Number of reflections	15062
<I/σ(I)>	15.76	2.91
Completeness [%]	99.2	99.86
Redundancy	4.9	3.84
CC(1/2)	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1 M Tris pH 8.5, 10% ethanol