5LPZ

Crystal structure of the BRI1 kinase domain (865-1196) in complex with ADP from Arabidopsis thaliana

Replaces:  4OA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918380
Spacegroup name	P 62
Unit cell lengths	116.833, 116.833, 50.558
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.470 - 2.480
R-factor	0.2255
Rwork	0.224
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uim
RMSD bond length	0.002
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	2.620
High resolution limit [Å]	2.480	2.480
Number of reflections	14064
<I/σ(I)>	22.43	2.63
Completeness [%]	98.6	92.5
Redundancy	20.02	18.66
CC(1/2)	1.000	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	37.5% MPD/PEG 1000/PEG 3350 morpheus mix, 0.1 M amino acids mix, 0.1 M MOPS/Hepes pH 7.5