Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 62 |
| Unit cell lengths | 116.137, 116.137, 50.295 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.356 - 2.431 |
| R-factor | 0.2057 |
| Rwork | 0.203 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uim |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.360 | 2.580 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 14560 | |
| <I/σ(I)> | 28.56 | 1.99 |
| Completeness [%] | 98.4 | 91.5 |
| Redundancy | 14.01 | 13.55 |
| CC(1/2) | 1.000 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 26% PEG 20K/MME 550 morpheus mix, 0.12 M eth glycol mix, 0.1 M Hepes pH 7.5 |
