5LPI
Structure of the zinc finger array of Cep104
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.149, 80.320, 118.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.246 - 1.800 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22940 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.809 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.310 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | |
| Number of reflections | 66109 | |
| <I/σ(I)> | 13.6 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 0.999 | 0.444 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 10 % PEG 4K 20% Glycerol 30 mM CaCl2 30 mM MgCl2 0.1M MES/imidazole pH 6.5 |
