5LPH
Structure of the TOG domain of human Cep104
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.94 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.288, 53.445, 155.356 |
| Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
| Resolution | 49.500 - 2.250 |
| R-factor | 0.23525 |
| Rwork | 0.234 |
| R-free | 0.25341 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0137) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.130 | |
| Number of reflections | 40229 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 0.997 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.1 M MES pH 6.0 11 % PEG 20K |
