5LOZ
STRUCTURE OF YEAST ENT1 ENTH DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.690, 35.460, 110.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.420 - 1.950 |
| R-factor | 0.193 |
| Rwork | 0.186 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.296 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.420 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 9844 | |
| <I/σ(I)> | 6.79 | 1.5 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20%(W/V) PEG 3350, 0.1 M HEPES PH 7.5, 3.5% TACSIMATE PH 7.0, VAPOR DIFFUSION, SITTING DROP |
