5LOJ

Structure of full length unliganded CodY from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93300
Spacegroup name	P 43 2 2
Unit cell lengths	111.524, 111.524, 119.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.940 - 3.710
R-factor	0.26905
Rwork	0.264
R-free	0.38942
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gx5 2b0l
RMSD bond length	0.018
RMSD bond angle	2.135
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.059	0.408
Number of reflections	8235
<I/σ(I)>	33.14	5.66
Completeness [%]	97.5	98.2
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	1.7M Li2SO4, sodium citrate pH 5.6, 3% dioxane