5LOA

Crystal structure of the engineered D-Amino Acid Dehydrogenase (DAADH) bound to NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	75.020, 64.580, 82.060
Unit cell angles	90.00, 106.89, 90.00

Refinement procedure

Resolution	49.877 - 1.840
R-factor	0.1734
Rwork	0.172
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.877
High resolution limit [Å]	1.840
Number of reflections	597464
<I/σ(I)>	10.5
Completeness [%]	99.4
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M sodium formate, 0.1 M Bis-Tris propane pH 8.5 and 20% PEG 3350