5LO7

Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9737
Spacegroup name	P 21 21 21
Unit cell lengths	41.980, 41.970, 150.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.681 - 1.900
R-factor	0.1623
Rwork	0.159
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lnd
RMSD bond length	0.012
RMSD bond angle	1.431
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	150.830	29.681	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.032	0.758
Rmeas	0.099	0.038	0.885
Rpim	0.049	0.019	0.447
Total number of observations	94487	3071	13603
Number of reflections	25547
<I/σ(I)>	9.6	20.4	1.8
Completeness [%]	99.9	97.4	100
Redundancy	3.7	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	35% dioxane v/v