5LNS

Crystal structure of Arabidopsis thaliana Pdx1-R5P complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9760
Spacegroup name	H 3
Unit cell lengths	176.728, 176.728, 114.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.786 - 1.910
R-factor	0.1595
Rwork	0.158
R-free	0.18860
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.800
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.940
High resolution limit [Å]	1.910	5.570	1.910
Rmerge	0.056	0.026	0.308
Number of reflections	103410
<I/σ(I)>	24.5
Completeness [%]	99.9	98.2	100
Redundancy	3.8	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH7.5, 0.5 M sodium citrate