5LNG
Lectin domain of E. coli F9 pilus adhesin FmlH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.710, 52.730, 62.820 |
| Unit cell angles | 66.90, 82.28, 84.10 |
Refinement procedure
| Resolution | 57.480 - 2.090 |
| R-factor | 0.19924 |
| Rwork | 0.197 |
| R-free | 0.23854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.846 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.073 | 0.547 |
| Number of reflections | 35660 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 97.8 | 96.8 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Citrate pH2, 1M Ammonium sulfate |
