5LNB
Crystal structure of the de-sumoylating protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.0644 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.540, 55.580, 172.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.390 - 2.300 |
| R-factor | 0.1607 |
| Rwork | 0.157 |
| R-free | 0.19680 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.320 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.126 | 0.677 |
| Number of reflections | 36221 | |
| <I/σ(I)> | 33.82 | 3.44 |
| Completeness [%] | 97.4 | 77.1 |
| Redundancy | 44.9 | 16.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M di-ammonium hydrogen citrate, 20 % PEG 3350, protein concentration 3 mg/ml |
