5LN5
Crystal structure of the Wss1 E203Q mutant from S. pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.160, 57.260, 50.640 |
| Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.750 |
| R-factor | 0.17404 |
| Rwork | 0.173 |
| R-free | 0.19541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jig |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.041 | 0.431 |
| Number of reflections | 20977 | |
| <I/σ(I)> | 14.1 | 2.7 |
| Completeness [%] | 95.3 | 94.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM TRIS/HCl, 20% PEG 1000 |
