5LM9
Structure of E. coli NusA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.688, 147.182, 52.387 |
| Unit cell angles | 90.00, 112.56, 90.00 |
Refinement procedure
| Resolution | 46.949 - 2.143 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2f |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.638 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 28443 | |
| <I/σ(I)> | 12.15 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES, pH 7.5, 0.1 M NaCl, 1.6 M (NH4)2SO4 |
