5LM2
Crystal Structure of HD-PTP phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.490, 47.650, 172.710 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 43.242 - 2.540 |
| R-factor | 0.2186 |
| Rwork | 0.215 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.516 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.240 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.110 | 0.780 |
| Number of reflections | 28609 | |
| <I/σ(I)> | 19.3 | 3.84 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 13.5 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Bis-Tris pH 6.0, 0.1-0.2 M Na-Formate and 13-15% PEG3350 |
