5LM1

Crystal Structure of HD-PTP phosphatase in complex with UBAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.930, 93.270, 102.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.820 - 2.550
R-factor	0.2151
Rwork	0.213
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INHOUSE
RMSD bond length	0.002
RMSD bond angle	0.397
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.820	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.087	0.760
Number of reflections	15835
<I/σ(I)>	13.55	2.51
Completeness [%]	100.0	100
Redundancy	6.3	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M KSCN, 20% PEG3350