5LLY

Photosensory Module of Bacteriophytochrome linked Diguanylyl Cyclase from Idiomarina species A28L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-17
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9660
Spacegroup name	P 1 21 1
Unit cell lengths	83.500, 129.000, 122.010
Unit cell angles	90.00, 96.34, 90.00

Refinement procedure

Resolution	48.133 - 2.400
R-factor	0.18
Rwork	0.177
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5llw
RMSD bond length	0.008
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.150		2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.071	0.025	0.650
Number of reflections	93405
<I/σ(I)>	10.31	31.23	1.47
Completeness [%]	93.0	77	94.1
Redundancy	2.85
CC(1/2)	0.997	0.998	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1 M Bis-Tris pH 5.5, 0.1 M ammonium acetate, 17 % (w/v) PEG 10,000