5LLK

Crystal structure of human alpha-dystroglycan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	H 3
Unit cell lengths	71.520, 71.520, 144.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.760 - 1.800
R-factor	0.1649
Rwork	0.163
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u2c
RMSD bond length	0.010
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2328: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.046	1.080
Number of reflections	25220
<I/σ(I)>	21.6	1.61
Completeness [%]	99.0	94.5
Redundancy	7	6.9
CC(1/2)	1.000	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.8 M Sodium Citrate