5LLB
Structure of Polyphosphate Kinase 2 from Francisella tularensis with AMPPCH2PPP and polyphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.700, 144.910, 70.590 |
| Unit cell angles | 90.00, 113.04, 90.00 |
Refinement procedure
| Resolution | 72.460 - 1.920 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3czq |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.460 |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.061 |
| Number of reflections | 82008 |
| <I/σ(I)> | 13.6 |
| Completeness [%] | 99.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20 % PEG 1500, pH 8 PCTP buffer, 0.5 mM polyP, 5 mM AMPPCH2P, 10 mM MgCl2 |
