5LKQ
Protease domain of RadA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 88.457, 88.457, 127.506 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.003 - 2.498 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.24450 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2474: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.192 | 2.260 |
| Rmeas | 0.236 | 2.783 |
| Rpim | 0.135 | 1.597 |
| Number of reflections | 12915 | |
| <I/σ(I)> | 9.9 | 2.5 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 5.8 | 5.6 |
| CC(1/2) | 0.992 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 9% PEG4000, 100 mM Tris pH 8 |
