5LKK
Protruding domain of GII.17 norovirus Kawasaki308 in complex with 3-fucosyllactose (3FL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.140, 86.710, 96.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.897 - 1.490 |
R-factor | 0.1328 |
Rwork | 0.131 |
R-free | 0.16390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fmo |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.900 | 1.520 | |
High resolution limit [Å] | 1.480 | 6.620 | 1.480 |
Rmerge | 0.074 | 0.047 | 0.795 |
Number of reflections | 103757 | ||
<I/σ(I)> | 20.85 | 45.7 | 3.8 |
Completeness [%] | 98.6 | 99.2 | 94.3 |
Redundancy | 13.6 | ||
CC(1/2) | 0.999 | 0.997 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Magnesium Chloride, PEG8000, Tris-HCl |