5LK3

Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 500 mM KCL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	H 3
Unit cell lengths	107.141, 107.141, 127.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.520 - 1.500
R-factor	0.1726
Rwork	0.171
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz
RMSD bond length	0.013
RMSD bond angle	1.391
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.520	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.095	0.530
Number of reflections	87160
<I/σ(I)>	8	2
Completeness [%]	99.6	99.4
Redundancy	4.2	3.9
CC(1/2)	0.989	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES 6.5, 10.77%(v/v) PEG 8000, 7% (v/v) glycerol, 500 mM KCl